Preview
Hüseyin Akbulut, MSc (2026). 300m Shuttle Run Test — Protocol, Norms and Application. Sporeus. Retrieved, October 9, 2026. https://sporeus.com/en/egzersiz-testleri-en/300m-shuttle-run-test/
Measures: Anaerobic capacity, glycolytic lactate tolerance, repeated-sprint recovery
Table of Contents
Difficulty: Hard | Category: Anaerobic
Protocol
The 300m Shuttle Run Test is a field test used to measure anaerobic capacity and lactate tolerance. The participant runs 25 meters back and forth 6 times (6×25m×2 = 300m) in the shortest possible time. Preparation: A flat, firm 25-meter surface is marked with two cones. The participant warms up with a 10-minute easy run and dynamic stretching. Body weight and height measurements are recorded. Test Procedure: The participant stands ready beside the starting cone. On command, they run to the 25-meter cone, touch it, and return. This cycle is repeated 6 times (6 complete round trips = 300m). Rather than coming to a full stop at each turn, the participant should change direction and continue. Total time is measured with a stopwatch. Second Trial: The standard includes 5 minutes of passive rest between two trials. Either the average of two trials or the best value may be used. The difference between trials (difference index) is a fatigue indicator. Data Recording: Each trial time is recorded in seconds. Difference index = (trial2 − trial1) / trial1 × 100. A high difference index indicates insufficient recovery capacity. Post-Test: Active cool-down is performed. Results are compared to normative tables by position and sport.Formula
Difference index = (trial2 - trial1) / trial1 × 100; lower time = higher capacity
Equipment Required
50 m flat area or track, cones, stopwatch
Normative Values (Age: 18-30 (team-sport athletes))
| Classification | Male | Female |
|---|---|---|
| Excellent | <54 sec | <60 sec |
| Good | 54-58 sec | 60-65 sec |
| Average | 58-63 sec | 65-71 sec |
| Below Average | 63-68 sec | 71-77 sec |
| Poor | >68 sec | >77 sec |
Practical Applications
This test is used in pre-season anaerobic conditioning assessment of football, basketball, and handball athletes, and to determine recovery capacity during intensive match schedules.Limitations
Without a photoelectric cell, measurement error can be high. Surface type (grass, tartan, hardwood) affects times; standardization is required.Related Tests: RAST Test, Repeated Sprint Ability Test, Wingate Test
Sources: Drust B et al., 1998, J Sports Sci; Reilly T & White C, 2005, J Hum Mov Stud
References
- Moore A, Murphy A. (2003). Development of an anaerobic capacity test for field sport athletes. Journal of Science and Medicine in Sport, 6(3): 275-284. doi:10.1016/S1440-2440(03)80021-X
- Bar-Or O. (1987). The Wingate anaerobic test: an update on methodology, reliability and validity. Sports Medicine, 4(6): 381-394. doi:10.2165/00007256-198704060-00001
Hüseyin Akbulut is the founder of Sporeus and author of THRESHOLD (EŞİK), a 540-page Turkish-language book on endurance science.
- 540pp THRESHOLD Book
- MSc Sport Sciences
- Marmara University
Protocol The 300m Shuttle Run Test is a field test used to measure anaerobic capacity and lactate tolerance. The participant runs 25 meters back and forth 6 times (6×25m×2 = 300m) in the shortest possible time. Preparation: A flat, firm 25-meter surface is marked with two cones. The participant warms up with a 10-minute easy run and dynamic stretching. Body weight and height measurements are recorded. Test Procedure: The participant stands ready beside the starting cone. On command, they run to the 25-meter cone, touch it, and return. This cycle is repeated 6 times (6 complete round trips = 300m). Rather than coming to a full stop at each turn, the participant should change direction and continue. Total time is measured with a stopwatch. Second Trial: The standard includes 5 minutes of passive rest between two trials. Either the average of two trials or the best value may be used. The difference between trials (difference index) is a fatigue indicator. Data Recording: Each trial time is recorded in seconds. Difference index = (trial2 − trial1) / trial1 × 100. A high difference index indicates insufficient recovery capacity. Post-Test: Active cool-down is performed. Results are compared to normative tables by position and sport. Formula
Difference index = (trial2 - trial1) / trial1 × 100; lower time = higher capacity
Equipment Required
50 m flat area or track, cones, stopwatch